Although neural tissue cancers are frequently regarded as terminal, the precise origins and molecular or cellular mechanisms underlying these conditions remain elusive. Despite existing knowledge, the progression patterns and secondary effects associated with different brain tumors have not yet been fully mapped out. Cerebrospinal fluid (CSF) leakage remains a prevalent diagnostic indicator in tumor detection, and in this context, enzymes associated with brain cancer were analyzed. The primary objective of this investigation was to explore how CSF is involved in brain cancer processes and its relationship with the blood-brain barrier (BBB). The study investigated common leakage points, particularly in the thoracic spine region and at the cranial base near the cardiothoracic interface, through a systematic mapping approach. The presence of bacteria in the CSF of brain cancer patients was also investigated, aiming to identify widely used strategies for targeting cancerous cells and detecting enzymes within CSF. Further insights pinpoint specific regions where leakage occurs and highlight particular proteins and enzymes implicated in this pathological process, showing how the discharge of tumor by-products contributes to CSF damage. Consequently, this research enabled the identification of enzymes and tumor cells in CSF and introduced a novel component that characterizes tumor-associated cerebrospinal fluid.
