TY  - JOUR
T1  - Methylation of cg24067911 Inhibits Colorectal Cancer Metastasis via the BCL6–ATXN1–CDH1 Pathway
A1  - Luis Felipe Vargas
A1  - Ricardo Jose Torres
JF  - Archive of International Journal of Cancer and Allied Science
JO  - Arch Int J Cancer Allied Sci
SN  - 3108-4834
Y1  - 2022
VL  - 2
IS  - 1
DO  - 10.51847/QEoZbYMsKw
SP  - 106
EP  - 121
N2  - DNA methylation critically influences colorectal cancer (CRC) development and metastasis, yet the precise effects at individual genomic sites are not fully elucidated. Previously, we found that hypomethylation at cg24067911 correlates with poor outcomes in CRC patients. To clarify the role of cg24067911 in metastatic progression, we used the CRISPR-dCas9-DNMT3a system to selectively modify its methylation. Integrating transposase-accessible chromatin sequencing, RNA sequencing, and chromatin immunoprecipitation revealed that cg24067911 lies within an ATXN1 enhancer and serves as a binding site for BCL6. Reduced methylation at this locus strengthened BCL6 interaction, boosting ATXN1 expression. In turn, ATXN1 functions as a transcriptional regulator of CDH2, driving epithelial-mesenchymal transition and enhancing metastatic potential in CRC. These findings uncover a novel epigenetic pathway—the BCL6–ATXN1–CDH2 axis—through which cg24067911 methylation impacts metastasis, highlight its prognostic value, and propose targeted epigenetic editing as a potential therapeutic approach to limit CRC spread.
UR  - https://smerpub.com/article/methylation-of-cg24067911-inhibits-colorectal-cancer-metastasis-via-the-bcl6atxn1cdh1-pathway-i5vq2q5wpj5qfpx
ER  -