The detrimental effect of vibration, which is considered one of the main physical factors in production, requires comprehensive scientific exploration. This study investigates how whole-body vibration influences protein metabolism using laboratory animals as a model. Two series of experiments were conducted involving a total of 30 white rats housed under uniform environmental conditions. Animals in group 1 were subjected to vibration exposure, whereas group 2 served as the control and did not experience any such exposure. The vibration parameters applied to group 1 included general vertical sinusoidal vibration at a frequency of 20 Hz and an intensity of 126 dB, administered for 4 hours per day for 8 weeks. The experimental data showed that while the total protein concentrations remained largely unaltered, a significant decrease in albumin levels was observed in the protein fraction profile. In addition, there was a significant elevation in both α-globulin and γ-globulin fractions. By the conclusion of the study, a statistically significant decline in serum concentrations of several total amino acids was detected in the vibration-exposed group, including aspartic acid (P < 0.05), proline (P < 0.05), glycine (P < 0.01), valine (P < 0.05), methionine (P < 0.05), and phenylalanine (P < 0.001). A general downward trend was also observed in the levels of hydrophobic (nonpolar) amino acids—specifically valine, proline, phenylalanine, and methionine—as well as in slightly polar uncharged and negatively charged amino acids such as aspartic acid.
